Figure 3
From: The E3 ubiquitin ligase TRIM62 and inflammation-induced skeletal muscle atrophy
Trim62 expression is increased during inflammation-, denervation- and starvation-induced muscle atrophy. (A) Quantitative RT-PCR (qRT-PCR) analyses of Trim62 expression in gastrocnemius/plantaris and tibialis anterior muscles at 24 hours, 48 hours, 72 hours and 96 hours after surgery, as indicated. CLP, Cecal ligation and puncture surgery. (B) qRT-PCR analyses of Trim62 expression in gastrocnemius/plantaris and tibialis anterior muscles 7 days, 14 days and 21 days after denervation or sham surgery. (C) qRT-PCR analyses of Trim62 expression in gastrocnemius/plantaris and tibialis anterior muscles of control mice (-, n =6) and mice deprived of food for 24 hours (n =6) or 48 hours (n =6), as indicated. Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) expression was used as the reference in all assays. Data shown are fold changes of expression in sham-operated and untreated mice. Data are presented as mean ± SEM. **P <0.01, *P <0.05.