Figure 2From: The E3 ubiquitin ligase TRIM62 and inflammation-induced skeletal muscle atrophyInflammation leads to skeletal muscle atrophy in vivo . Six- to eight-week-old male C57BL/6 N mice were subjected to sham operations (sham, n =5) or cecal ligation and puncture (CLP) surgery (n =5), as indicated, to induce polymicrobial sepsis. (A) Weights of the gastrocnemius/plantaris and tibialis anterior muscles were determined after 24 hours, 48 hours, 72 hours or 96 hours and normalized to tibia length. Data are presented as mean ± SEM. ***P <0.001, *P <0.05. Quantitative RT-PCR analyses of Atrogin1 (B) and MuRF1 (C) expression, and immunoblotting of proteins using anti-MuRF1 antibody (D), in gastrocnemius/plantaris and tibialis anterior muscles at 24 hours, 48 hours, 72 hours or 96 hours after surgery, as indicated. Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) expression and protein content were used as reference values, and data shown are relative changes. Data are presented as mean ± SEM. ***P <0.001, **P <0.01, *P <0.05. MuRF1, Muscle RING (really interesting new gene) finger-containing protein 1.Back to article page