Figure 5
From: Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation
Histone effects after toll-like receptor 4 (TLR4) blockade. Blocking antibody control was obtained from animals housed under the same condition as immunized animals to produce TLR4 antibody. (A) Percentage of propidium iodide (PI)-positive human microvascular endothelial cells (HMEC) after 24 hours stimulation with 50 μg/ml histones with and without blocking of TLR4. ***Student's t-test, P <0.001. (B) Lactate dehydrogenase (LDH) release significantly decreased after blocking with specific TLR4 antibody compared to histone-stimulated cells. **Student's t-test, P <0.001.