Figure 3
From: Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation
Evaluation of cellular integrity and viability after histone stimulation of endothelial and monocytic cells. (A) Lactate dehydrogenase (LDH) levels significantly increased in supernatant of human microvascular endothelial cells (HMEC) after 50 μg/ml histones stimulation for 24 h. ***Student's t-test, P <0.001. (B) LDH levels significantly increased in supernatant of mono mac 6 (MM6) cells after 50 μg/ml histones stimulation for 24 h. ***Student's t-test, P <0.001. (C) Percentage of propidium iodide (PI)-positive HMEC cells significantly increased after 24 hours stimulation with 50 μg/ml histones. *Student's t-test, P <0.05. (D) Percentage of PI-positive MM6 cells significantly increased after 24 hours stimulation with 50 μg/ml histones. ***Student's t-test, P <0.001.