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Figure 6 | Critical Care

Figure 6

From: Endothelial dysfunction and renal fibrosis in endotoxemia-induced oliguric kidney injury: possible role of LPS-binding protein

Figure 6

Prevention of LPS-induced renal fibrosis by CPFA treatment. Renal biopsies after CPFA treatment showed a significant reduction in collagen deposits (A, thin arrow), in particular along capillaries (zoomed image), and glomerular thrombi (thick arrow) compared with endotoxemic condition. Magnification 6±. (C) Immunofluorescence analysis showed a strong expression of interstitial α-SMA (green) in endotoxemic pigs that was dramatically decreased by CPFA treatment (white arrow). Magnification, 630×. To-pro 3 was used to counterstain nuclei (blue). (E) Caspase-3 staining highlighted the efficacy of treatment (black arrows) in reversing LPS-induced tubular apoptosis. Magnification, 14×. The quantitative analyses of Masson trichrome (B), α-SMA (D), and caspase-3 (F) staining were obtained as described in the Methods section and expressed as median ± IQR of at least five independent pigs for each group.

Prevention of LPS-induced renal fibrosis by CPFA treatment. Renal biopsies after CPFA treatment showed a significant reduction in collagen deposits (A, thin arrow), in particular along capillaries (zoomed image), and glomerular thrombi (thick arrow) compared with endotoxemic condition. Magnification 6±. (C) Immunofluorescence analysis showed a strong expression of interstitial α-SMA (green) in endotoxemic pigs that was dramatically decreased by CPFA treatment (white arrow). Magnification, 630×. To-pro 3 was used to counterstain nuclei (blue). (E) Caspase-3 staining highlighted the efficacy of treatment (black arrows) in reversing LPS-induced tubular apoptosis. Magnification, 14×. The quantitative analyses of Masson trichrome (B), α-SMA (D), and caspase-3 (F) staining were obtained as described in the Methods section and expressed as median ± IQR of at least five independent pigs for each group.

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