Table 1 Antibodies and processing used in immunohistochemical analyses
From: Small airway remodeling in acute respiratory distress syndrome: a study in autopsy lung tissue
Antibody | Pre-treatment | Specie | Clone | Dilution | Origin |
|---|---|---|---|---|---|
Collagen I | Citrate | Goat | Polyclonal | 1:1000 | US Biological-Swampscott, Massachusetts/USA |
Collagen III | Trypsin | Mouse | III-53 | 1:100 | Oncogene & Calbiochem, Darmstadt/Germany |
Fibronectin | Citrate | Rabbit | Polyclonal | 1:6000 | Dako, Glostrup/Denmark |
Versican | Trypsin | Mouse | 2-B-1 | 1:100 | Seikagaku CO, Tokyo/Japan |
MMP-2 | Citrate | Mouse | A-Gel VC2 | 1:3000 | LabVision, Fremont/USA |
MMP-9 | Citrate | Mouse | 56-2A4 | 1:50 | Calbiochem, Darmstadt/Germany |