Cell numbers in blood (A), spleen (B) and thymus (C). Mice underwent a sham procedure, CLP, CLP plus anti-PD-L1 administration, or CLP plus isotype administration (n = 5 for each group). Blood, thymus and spleen were harvested 24 h after surgery. The total cell number was counted after lysis of erythrocytes (for spleen and thymus, preparation of a single-cell suspension was required). Lymphocyte numbers (CD3+ T cells, CD3- T cells, CD19+ B cells) were calculated by the total number and percent of lymphocyte subgroups resulting from FACS analysis, respectively. * P <0.05.