Figure 1

NF-κB signaling. Levels of phosphorylated NF-κB p65 (pNF-κB) in lymphocytes (Ly) of healthy subjects (circles) and patients (quadrangles) with acute pancreatitis were measured in whole blood samples left without supplement (open symbols) or supplemented (closed symbols) with TNF (10 ng/ml, five minutes), E. coli, S. aureus, S. epidermidis, LPS, or MDP. Responses to TNF, determined as: A. Fluorescence intensity (FI) of all Ly and subsets of Ly (N = 9 to 10); B. As a proportion of pNFκB-positive Ly and their FI. C. The sample histograms of TNF-stimulated (white) and non-stimulated (gray) Ly. The M1 (marker) denotes proportion of pNF-κB-positive Ly. D. Responses to bacteria, LPS, and MDP, determined as the FI of all Ly (N = 8 to 10). E. As proportion of pNFκB-positive cells among all Ly and their FI. RFU, relative fluorescence units. In A and D, horizontal lines denote median, and in B and E, data are given as mean ± SEM. *P < 0.05, **P < 0.01, *** P < 0.001.