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Table 2 Effects of PMN-activation on selectin function at 2 dyne/cm2

From: Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injury

 

Adhesion [PMN/mm2]

Blocking antibody

HUVEC++/PMN-

HUVEC++/PMN++

HUVEC++/PMN++/PLT++

NONE

1042 ± 61

591 ± 43

1313 ± 25

L-

744 ± 67 *

607 ± 56 ns vs NONE

Ø

P-

833 ± 59 ns vs NONE

596 ± 85 ns vs NONE

396 ± 35 * vs NONE

E-

504 ± 55 * vs NONE

267 ± 32 * vs NONE

Ø

L-/P-

674 ± 48 ns vs L-

Ø

Ø

E-/P-

504 ± 91

230 ± 12 ns vs E-

Ø

L-/E-

405 ± 59 * vs L-

Ø

Ø

L-/E-/P-

343 ± 40

Ø

Ø

  1. Adhesion in lipopolysaccharide-activated cultures (100 ng/ml; HUVEC++, PMN++, PLT++) at 2 dyne/cm2. Ø (not determined); * and ns (P < 0.05 versus indicated group or not significant, respectively). Statistical analysis with paired t-tests and correction after Bonferroni-Holm (mean ± SEM; n = 4).
  2. For comparison, background adhesion in non-activated cultures (HUVEC-/PMN-) at 2 dyne/cm2 revealed 247 ± 52 PMN/mm2.
  3. HUVEC, human umbilical venous endothelial cells; PMN, polymorphonuclear neutrophils; PLT, platelets; L-, leukocyte selectin; P-, platelet selectin; E-, endothelial selectin; SEM, standard error of the mean.