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Table 2 Effects of PMN-activation on selectin function at 2 dyne/cm2

From: Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injury

  Adhesion [PMN/mm2]
Blocking antibody HUVEC++/PMN- HUVEC++/PMN++ HUVEC++/PMN++/PLT++
NONE 1042 ± 61 591 ± 43 1313 ± 25
L- 744 ± 67 * 607 ± 56 ns vs NONE Ø
P- 833 ± 59 ns vs NONE 596 ± 85 ns vs NONE 396 ± 35 * vs NONE
E- 504 ± 55 * vs NONE 267 ± 32 * vs NONE Ø
L-/P- 674 ± 48 ns vs L- Ø Ø
E-/P- 504 ± 91 230 ± 12 ns vs E- Ø
L-/E- 405 ± 59 * vs L- Ø Ø
L-/E-/P- 343 ± 40 Ø Ø
  1. Adhesion in lipopolysaccharide-activated cultures (100 ng/ml; HUVEC++, PMN++, PLT++) at 2 dyne/cm2. Ø (not determined); * and ns (P < 0.05 versus indicated group or not significant, respectively). Statistical analysis with paired t-tests and correction after Bonferroni-Holm (mean ± SEM; n = 4).
  2. For comparison, background adhesion in non-activated cultures (HUVEC-/PMN-) at 2 dyne/cm2 revealed 247 ± 52 PMN/mm2.
  3. HUVEC, human umbilical venous endothelial cells; PMN, polymorphonuclear neutrophils; PLT, platelets; L-, leukocyte selectin; P-, platelet selectin; E-, endothelial selectin; SEM, standard error of the mean.