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Figure 4 | Critical Care

Figure 4

From: Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injury

Figure 4

Interdependent effects of shear stress and cell activation on PMN rolling. Rolling of neutrophils under different activation protocols (mean ± SEM; n = 4), (a) non-activated controls, (b) activation with 100 ng/ml LPS and (c) activation with 10 ng/ml LPS. Blank symbols indicate activated PMN, filled symbols indicate non-activated PMN. (d-f) show the corresponding curves for predicted rolling fractions determined by ANCOVA of logarithms (continuous line: non-activated PMN, discontinuous line: activated PMN). Rolling increased with decreasing shear stress in all cultures (a-c). On non-activated (d) and sub-maximal activated HUVEC (f) decreased shear stress increased the rolling fraction (P < 0.05, ANCOVA) whereas it had no effect under maximal LPS-activation (e). Activation of PMN induced higher rolling fractions in comparison to non-activated PMN at sub-maximal activation ((f), P < 0.05, ANCOVA).

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