Figure 4From: Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injuryInterdependent effects of shear stress and cell activation on PMN rolling. Rolling of neutrophils under different activation protocols (mean ± SEM; n = 4), (a) non-activated controls, (b) activation with 100 ng/ml LPS and (c) activation with 10 ng/ml LPS. Blank symbols indicate activated PMN, filled symbols indicate non-activated PMN. (d-f) show the corresponding curves for predicted rolling fractions determined by ANCOVA of logarithms (continuous line: non-activated PMN, discontinuous line: activated PMN). Rolling increased with decreasing shear stress in all cultures (a-c). On non-activated (d) and sub-maximal activated HUVEC (f) decreased shear stress increased the rolling fraction (P < 0.05, ANCOVA) whereas it had no effect under maximal LPS-activation (e). Activation of PMN induced higher rolling fractions in comparison to non-activated PMN at sub-maximal activation ((f), P < 0.05, ANCOVA).Back to article page