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Figure 1 | Critical Care

Figure 1

From: Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injury

Figure 1

Parallel plate flow chamber. The flow chamber consisted of a heatable metal case (1). The silicone-sealed coverslips (2) were placed in the middle. Using a transparent cover block (3) with a flow channel (4) and a scaled metal ring (5), the chamber could be closed to a defined height leaving an inner chamber with a defined height of 0.2 mm. The tubing of the cell suspension was connected by a needle (6) to the inlet and outlet port of the transparent cover block (4). Temperature was controlled by temperature measurement within the metal case. Preliminary experiments showed that temperature of the metal block equaled with temperature of the perfusate within few seconds. For microscopy of the adhesion assay, the whole system was placed on an inverted phase-contrast microscope.

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