Figure 3
From: Increased HMGB1 expression and release by mononuclear cells following surgical/anesthesia trauma
Analysis of IL-6 levels. (a) Analysis of IL-6 levels in the supernatants of monocytes from the patients under test. Monocytes were incubated in the presence or in the absence of 100 ng/ml HMGB1 or 100 ng/ml LPS plus 100 ng/ml HMGB1 for 24 h at 37°C. The samples were collected and analyzed using a commercially available enzyme-linked immunosorbent assay kit. Values are plotted as mean ± SD. ***HMGB1 vs control: P < 0.001; LPS vs control: P < 0.001; LPS plus HMGB1 vs control: P < 0.001. (b) Analysis of IL-6 levels in serum samples from the patients at three different times: t0 = before surgery, t1 = immediately after surgical procedure; t2 = at 24 hours following intervention. Sera from healthy subjects served as controls. The samples were collected and analyzed using a commercially available enzyme-linked immunosorbent assay kit. Values are plotted as mean ± SD. **t2 vs t0 : P = 0.006, t2 vs t1: P = 0.003. t1 vs t0: NSS.