Figure 2
From: Increased HMGB1 expression and release by mononuclear cells following surgical/anesthesia trauma
HMGB1 serum concentration. (a) Western blot analysis of serum HMGB1 concentration. Serum samples, obtained from the patients at three different times: t0 = before surgery, t1 = immediately after surgical procedure; t2 = at 24 hours following intervention, were analyzed by Western blot for reactivity with anti-human HMGB1 MoAb (1 μg/ml). A representative patient is shown together with a control serum from a healthy donor. (b) Densitometric analysis of serum HMGB1 concentration was revealed by Western blot at three different times: t0 = before surgery, t1 = immediately after surgical procedure; t2 = at 24 hours following intervention (arbitrary units). (c) Values of densitometric analyses of all the patients under test are shown as mean ± SD (arbitrary units). ***t2 vs t0, t2 vs t1: P < 0.001. t1 vs t0: NSS.