Figure 4

Urine, serum, and renal IL-6 in pre-renal azotemia and ischemic AKI. (A) Urine IL-6 increases in mice with ischemic AKI, but not pre-renal azotemia. Spontaneously voided urine was collected at baseline and from zero to two hours, two to four hours, and four to six hours after vehicle-injection (Veh), furosemide injection/pre-renal azotemia (Pre), sham operation (Sham) and ischemic AKI (AKI). Urine IL-6 was increased at four to six hours after ischemic AKI; median and SD (*P < 0.01 vs. Veh, Pre, Sham, n = 5 to 7). (B) Serum IL-6 increases in mice with ischemic AKI prior to the increase in urine IL-6. Serum IL-6 was determined at baseline, and two, four and six hours after vehicle-injection (Veh), furosemide injection/pre-renal azotemia (Pre), sham operation (Sham) and ischemic AKI (AKI) and was significantly increased at two, four and six hours after AKI (P < 0.01 vs. Veh, Pre, Sham at all time points; n = 4 to 11). (C) Kidney IL-6 increases in mice with ischemic AKI prior to the increase in urine IL-6. Kidney IL-6 was determined at baseline, and two, four and six hours after vehicle-injection (Veh), furosemide injection/pre-renal azotemia (Pre), sham operation (Sham) and ischemic AKI (AKI) and was significantly increased at two, four and six hours after AKI (P < 0.01 vs. Veh, Pre, Sham at all time points; n = 3 to 7).