Figure 1

TLR4 and injury responsive (TIR) genes selection criteria. (a) Genes that were significantly differentially expressed (P- value < 0.05 and ≥1.2-fold change) in PBL obtained from subjects challenged with in vivo endotoxin (Endo) for six hours (2 ng/kg), subjects infused with cortisol (Cort) (3 μg/kg/min) for 12 hours starting 6 hours before endotoxin administration (Cort + Endo), or from trauma patients PBL obtained within the initial five days after ICU admission, as compared to baseline, were identified. The Venn diagram identifies the genes that are common between groups. Nine hundred thirty-seven (937) genes were common to all three groups. (b) Scatter plot analysis comparing Group 1 genes expression trends between the indicated groups. (c) Genes that were significantly differentially expressed in trauma patients PBL obtained within 9 to 12 days after ICU admission as compared to baseline were identified. (d) Four hundred and forty-five genes were differentially expressed in both in vivo endotoxin challenged PBL and in PBL obtained from trauma patients over a period of 1 to 12 days after admission.