Flagellin induces NF-κB-DNA binding in the lung, liver, gut and kidney. (a) Nuclear factor (NF)-κB-DNA binding activity (electromobility shift assay (EMSA)) in mouse tissues 30 minutes after flagellin (1 μg/mouse) or saline (sham). (b) Supershift experiments. Nuclear proteins were incubated with an anti-p50 or an anti-p65 antibody prior to incubation with the radioactive probe. In all organs, the shifted bands supershifted in the presence of anti-p65. A large shifted band, which was not affected by anti-p65 and anti-p50 was detected in the liver. Densitometric analysis, * P < 0.05 flagellin vs sham, t-test (n = 5 mice/group).