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Table 1 Quantitative reverse-transcription PCR performance, parameters and primers

From: Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients

Gene symbol UniGene Efficiencya Errorb Primer sequencesc
BCL2 Hs.150749 1.87 0.0059 5'-GCGACGACTTCTCCCGC-3'
     5'-GCGATGTTGTCCACCAGG-3'
BCL-XL Hs.516966 1.89 0.0101 5'-GTAGTGAATGAACTCTTCCG-3'
     5'-GTATCCCAGCCGCCGTTCTC-3'
BAX Hs. 624291 1.75 0.0297 5'-CAAACTGGTGCTCAAGG-3'
     5'-CCAACCACCCTGGTCTTGG-3'
BAK Hs.485139 1.91 0.0175 5'-GCCACCAGCCTGTTTGAG-3'
     5'-CTGCCACCCAGCCACCC-3'
BIM Hs.469658 1.95 0.0112 5'-GAGCCACAAGACAGGAGC-3'
     5'-CCATTGCACTGAGATAGTGG-3'
BID Hs.591054 1.69 0.0184 5'-TGGTGTTTGGCTTCCTCCAA-3'
     5'-GAATCTGCCTCTATTCTTCCC-3'
FAS Hs.244139 1.93 0.0198 5'-GGAATCATCAAGGAATGCAC-3'
     5'-CCAAACAATTAGTGGAATTGG-3'
HLA-DRA Hs.520048 2.04 0.0195 5'-GCCAACCTGGAAATCATGACA-3'
     5'-AGGGCTGTTCGTGAGCACA-3'
  1. aAmplification efficiency of standard curve. bThe standard curve error value. cTop sequence is forward primer, bottom is reverse primer.