Figure 2

Flow cytometric measurement of Annexin-V binding, active caspase-3 and Bcl-2 expressions in lymphocyte subpopulations. (a) Percentage of cells binding Annexin-V, and the mean fluorescence intensities (MFIs) of (b) active caspase-3 and (c) Bcl-2 expressions were measured by flow cytometry in CD19⁺, CD4⁺ and CD8⁺ T-lymphocyte subpopulations of healthy volunteers (HV) (open boxes, n = 12) and septic shock patients (grey boxes) at days 1 to 2 (D1-2, n = 11) and days 3 to 5 (D3-5, n = 10). Data presented as boxplots. Comparison versus healthy volunteers values (means) performed using the Mann-Whitney U test with correction for the number of tests performed: *P < 0.025.