Hypervolemia induces and potentiates lung damage after recruitment maneuver in a model of sepsis-induced acute lung injury

Table 4 Semiquantitative analysis of electron microscopy

	HYPO		NORMO		HYPER
	NR	RM-CPAP	NR	RM-CPAP	NR	RM-CPAP
Alveolar capillary membrane	2 (2-2.5)	2 (2-3)	2 (2-2.25)	3 (2-3)	3**# (3-3.25)	4‡§ (3.75-4)
Type II epithelial cell	2 (2-2.25)	3 (2-3)	2 (2-2.25)	3 (2-3)	3 (2.75-4)	4‡§ (3.75-4)
Endothelial cell	2 (1.75-2.25)	2 (2-3)	2 (2-2.25)	3 (2.75-3)	3**# (3-4)	4‡§ (3.75-4)

Pathologic findings were graded according to a five-point semi-quantitative severity-based scoring system: 0 = normal lung parenchyma, 1 = changes in 1 to 25%, 2 = 26 to 50%, 3 = 51 to 75%, and 4 = 76 to 100% of the examined tissue. Animals were randomly assigned to hypovolemia (HYPO), normovolemia (NORMO) or hypervolemia (HYPER) with recruitment maneuver (RM-CPAP) or not (NR). Values are the median (25^th percentile to 75^th percentile) of five animals per group. **Significantly different from HYPO-NR (P < 0.05). ‡ Significantly different from HYPO-RM-CPAP (P < 0.05). #Significantly different from NORMO-NR (P < 0.05). §Significantly different from NORMO-RM-CPAP (P < 0.05).

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