Representative flow cytometry analysis of HLA-DR expression on CD14HIGH and CD14LOW monocyte subsets. Representative flow cytometry analysis of HLA-DR expression on CD14HIGH and CD14LOW monocyte subsets during and after surgery. (A) Flow cytometric analysis was performed on whole blood samples after elimination of neutrophils (N), lymphocytes (L) and red cells (R) using the forward scatter (FSC) and side scatter (SSC) characteristics. (B) A representative dot-plot showing that CD14HIGH monocytes were CD16- and CD14LOW monocytes were CD16+ in a patient before surgery. (C) Monocytes were analyzed using an anti-CD14 antibody coupled with phycoerythrin (PE) and an anti-HLA-DR antibody coupled with fluorescein isothiocyanate (FITC). Representative flow cytometric analysis for HLA-DR expression on CD14HIGH and CD14LOW monocytes performed before anesthesia (T1) and on postoperative Day 1 (POD1) are shown for two abdominal aortic surgery (AAS) patients.