Figure 2
From: Matrix-metalloproteinase-2, -8 and -9 in serum and skin blister fluid in patients with severe sepsis
MMP-2 and MMP -9 levels in suction blister fluids of patients with severe sepsis and healthy controls were measured by gelatin zymography. All the gels had matrix metalloproteinase (MMP)-2 and MMP-9 samples purified from fibroblast and keratinocyte mediums, respectively. (a) MMP-2 and MMP-9. Pro and active forms of MMP-2 and MMP-9 are shown by arrows. As the running time for different gels varied slightly the bands are not exactly at the same level in samples analyzed in different gels. Three different healthy control samples (C1, C2, C3) are shown together with purified control MMP-2 and MMP-9. (b) Samples from two different surviving patients (P1, P2) are shown. For each of them one and five days and three and six month samples were run side by side in the gel. (c) Samples from four different non-surviving patients (P 3 to P 6) on days one and five (run side by side in the gel for each of them) are shown. (d) Purified control MMP-2 and MMP-9 and two different patient samples (P7, P8) incubated with (+) or without (-)4-aminophenylmercuric acetate (APMA) are shown (each sample with and without APMA was run side by side in the gel). In samples with APMA activation the bands corresponding to the proforms are weakened. Asteriks indicates the intermediate sized MMP-2 or MMP-9.