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Figure 1 | Critical Care

Figure 1

From: Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathway

Figure 1

Timeline of the protocol. Animals were anesthetized, a tracheotomy was performed and animals were connected to a ventilator and ventilated in volume-controlled mode at 6 ml/kg, 5 cmH2O positive end-expiratory pressure. Arterial and venous catheters were inserted. One hour before lipopolysaccharide intratracheal injection, vehicle control or WW85 or PJ-34 were infused. At t = 0 minute, mechanical ventilation setting was changed according to the randomization and renal blood flow was measured. From t = 120 minute to t = 180 minutes urine was collected and blood samples were taken. At time t = 180 minutes renal blood flow was measured with different fluorescence microspheres. At the end of the experiment, at t = 210 minutes, blood samples were taken, animals were sacrificed, organs were harvested and arcuate renal arteries were isolated. Vt, tidal volume.

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Critical Care

ISSN: 1364-8535