Figure 6
From: Protective effect of resin adsorption on septic plasma-induced tubular injury
Effect of resin adsorption on septic plasma-induced alteration of polarity and expression of TEC transporters. (a) Evaluation of tubular epithelial cells (TEC) polarity expressed as trans-epithelial electrical resistance (TER). Septic plasma induced a significant decrease of TER (*P < 0.05 Septic vs. Healthy or Vehicle) that was inhibited by Amberchrom resin adsorption (§P < 0.05 Septic + CG161 M vs Septic). Data are expressed as average TER values (ohm/cm2) ± standard deviation. Results were normalized for the membrane area of transwell used in the test. Analysis of variance with Newmann-Keuls multicomparison test was performed. (b) Representative immunofluorescence micrographs of the expression of the tight junction protein zonula occludens-1 (ZO-1), the sodium channel NHE3 and the glucose transporter GLUT-2 in TEC cultured with control healthy plasma or septic plasma before and after (Septic + CG161 M) Amberchrom resin adsorption. Nuclei were counterstained by 1 μg/ml propidium iodide (magnification ×200).