Figure 1

Control data. After preparation, 14 days of cultivation and baseline fluorescence imaging, slices were either impaired with oxygen-glucose deprivation (OGD) or traumatic brain injury (TBI) (see panel A or B respectively). For OGD, the slices were incubated in glucose free medium and transferred into an airtight anoxic chamber where they were incubated in an atmosphere of 95% N₂ and 5% CO₂ for 30 minutes. TBI was induced by the impact of a stylus onto the CA1 region of the hippocampus. After trauma, the slices were transferred to an airtight chamber and incubated in an atmosphere of 21% O₂, 5% CO₂ and 74% N₂. The negative control groups' slices were subjected to the same treatment, except for the trauma. After 72 hours the damage was assessed by fluorescence imaging and pixel-based image analysis. In both panels, both curves labelled as a show the histogram of non-traumatized slices (OGD: n = 58 prepared from six mice; TBI: n = 35 prepared from six mice) after 72 hours. The middle line is the mean value; the upper and lower lines represent the upper and lower bounds of the SEM. Curves b present the histogram of traumatized slices (OGD: n = 71 prepared from eight mice; TBI: n = 39 prepared from six mice). The vertical dashed line is the applied threshold at a gray scale value of 100. The sum over all pixel values greater than this threshold were calculated for each group and defined as the trauma intensity. Inserts in panel A and B respectively present the controls normalized to the trauma groups.