Skip to main content

Table 1 Primers and endonucleases for genotyping of IL-10 promoter single nucleotide polymorphisms

From: Clinical relevance of the interleukin 10 promoter polymorphisms in Chinese Han patients with major trauma: genetic association studies

SNPs

Primers

PCR conditions

Restriction endonucleases

-1082A/G

F:ACACAAATCCAAGACAACACTACTAAGGCTTCCTTGGGA

3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 56°C, 40 seconds at 72°C, then 10 minutes at 72°C

XagI

 

R:GTGATCAAACAGAGGCACAGACAT

  

-819T/C

F:CACTCTAAGGCTTCCTTGGGA

3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 56°C, 40 seconds at 72°C, then 10 minutes at 72°C

Hin1α

 

R:CCTACCGTCTCTATTTTATAGTGAGCAAACTGAGGCACAGACAT

  

-592 A/C

F:AGCTGAAGAGGTGGAAACATGTG

3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 63°C, 40 seconds at 72°C, then 10 minutes at 72°C

Rsa I

 

R:TGGGTTCTCATTCGCGTGTT

 Â