Ventilator-induced endothelial activation and inflammation in the lung and distal organs

Table 1 Primers used for quantitative real-time RT-PCR

	Forward	Reverse
E-selectin	CAACgTCTAggTTCAAAACAATCAg	TTAAgCAggCAAgAggAACCA
VCAM-1	TgAAgTTggCTCACAATTAAgAAgTT	TgCgCAgTAgAgTgCAAggA
ICAM-1	ggAgACgCAgAggACCTTAACAg	CgACgCCgCTCAgAAgAACC
PECAM-1	ACgATgCgATggTgTATAAC	ACCTTgggCTTggATACg
IL-1β	CAACCAACAAgTgATATTCTCCATg	gATCCACACTCTCCAgCTgCA
TNF-α	gCggTgCCTATgTCTCAg	gCCATTTgggAACTTCTCATC
KC	AAAAggTgTCCCCAAgTAACg	gTCAgAAgCCAgCgTTCAC
β-actin	AgAgggAAATCgTgCgTgAC	CAATAgTgATgACCTggCCgT
GAPDH	TgAAgCAggCATCTgAggg	CgAAggTggAAgAgTgggAg

GAPDH = glyceraldehyde 3-phosphate dehydrogenase; ICAM = intercellular adhesion molecule; IL = interleukin; KC = keratinocyte-derived chemokine; PECAM = platelet-endothelial cell adhesion molecule; TNF = tumor necrosis factor; VCAM = vascular cell adhesion molecule.

ISSN: 1364-8535