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Figure 5 | Critical Care

Figure 5

From: DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model

Figure 5

TNF-α expression. (a) In liver after 48 and 96 hours. Relative mRNA expression of TNF-α in liver tissue, detected by semi-quantitative RT-PCR 48 and 96 hours after the second hit. The amount of the specific PCR product was quantified densitometrically. The values were normalized by calculating the quotient of the amount of TNF-α mRNA against the amount of mRNA of the housekeeping gene GAPDH. * P ≤ 0.05. The data are expressed as mean ± standard error of the mean. (b) In lung after 48 and 96 hours. Relative mRNA expression of TNF-α in lung tissue, detected by semi-quantitative RT-PCR 48 and 96 hours after the second hit. The amount of the specific PCR product was quantified densitometrically. The values were normalized by calculating the quotient of the amount of TNFα mRNA against the amount of mRNA of the housekeeping gene GAPDH. * P ≤ 0.05. The data are expressed as mean ± standard error of the mean. CLP = cecal ligation and puncture; DHEA = dehydroepiandrosterone; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.

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Critical Care

ISSN: 1364-8535