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Table 1 Experimental groups and procedure of animals per group

From: Unfractionated heparin and enoxaparin reduce high-stretch ventilation augmented lung injury: a prospective, controlled animal experiment

 

EBD

(5 hours)

Lung water, PAI-1

(5 hours)

Neutrophils, MPO

(5 hours)

PAI-1 mRNA

(1 hour)

IHC, H&E stain

(5 hours)

Control

6

6

6

6

6

VT6 ml/kg

6

6

6

6

6

VT30 ml/kg

6

6

6

6

6

VT30 ml/kg

+ 200 IU/kg UFH

6

6

6

6

6

VT30 ml/kg

+ 400 IU/kg UFH

6

6

6

6

6

VT30 ml/kg

+ 4 mg/kg enoxaparin

6

6

6

6

6

VT30 ml/kg

+ 8 mg/kg enoxaparin

6

6

6

6

6

VT30 ml/kg

+ PAI-1-/-

6

6

6

6

6

  1. Control = spontaneously breathing, nonventilated mice; EBD = Evans blue dye, a measurement of microvascular leak in the lung; H&E = hematoxylin and eosin; IHC = immunohistochemical stain; lung water = measured by lung wet- to dry-weight ratio; MPO = myeloperoxidase, a measurement of total neutrophil infiltration in the lung; PAI-1 = plasminogen-activator inhibitor-1; UFH = unfractionated heparin; VT = tidal volume. Time points for measurements were determined according to our previous findings that mediator activation occurs early in ventilator-induced lung injury, and neutrophil infiltration occurs later [14].
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Critical Care

ISSN: 1364-8535