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Figure 3 | Critical Care

Figure 3

From: Unfractionated heparin and enoxaparin reduce high-stretch ventilation augmented lung injury: a prospective, controlled animal experiment

Figure 3

Unfractionated heparin and enoxaparin reduced stretch-induced plasminogen activator inhibitor-1 (PAI-1) mRNA expression and protein production. The mice were ventilated at VT 6 ml or VT 30 ml for 1 hour with room air. Unfractionated heparin, 200 IU/kg or 400 IU/kg, and enoxaparin, 4 mg/kg or 8 mg/kg, was given subcutaneously 30 minutes before ventilation. (a) Reverse transcription-polymerase chain reaction (RT-PCR) assay was performed for PAI-1 mRNA (a, top panel), glyceraldehydes-phosphate dehydrogenase (GAPDH) mRNA (a, middle panel), and arbitrary units (a, bottom panel) (n = 6 per group). Arbitrary units were expressed as the ratio of PAI-1 mRNA to GAPDH. (b) Active PAI-1 production in BAL fluid. *P < 0.05 versus control, nonventilated mice; † P < 0.05 versus all other groups; ‡ P < 0.05 versus 200-IU/kg or 4-mg/kg groups. H = unfractionated heparin; LMWH = enoxaparin.

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