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Figure 4 | Critical Care

Figure 4

From: Propofol: neuroprotection in an in vitro model of traumatic brain injury

Figure 4

The extent of tissue trauma in the slices was quantified using pixel-based analysis of the acquired fluorescence images at t = 72 hours after trauma. Total injury was defined as the total level of cell death in the slices. A minimum of 12 slices were evaluated per group. Secondary injury was calculated by covering the pin's direct impact site in the images with a defined mask excluding this area from trauma analysis. (a) The concentration-response curves of propofol from 10 to 400 μM for both total (filled circles, upper curve) and secondary injury (open circles, lower curve). (b) Exemplary images for total and secondary injury (showing the impact site exclusion mask) in the control group and at a propofol concentration of 200 μM. Non-linear regression curves were fitted into the graph to visualise the trends.

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