Figure 2

After preparation, cultivation for 14 days and baseline measurement, slices were either traumatised or not by the impact of a stylus onto the CA1 region of the hippocampus. The extent of the trauma was evaluated by fluorescence imaging 72 hours after the induced trauma and pixel-based image analysis. Curve a shows the histogram of non-traumatised slices (n = 17) at t = 72 hours. The straight line is the mean value, the dashed lines are the upper and lower bounds of the standard error of the mean. Curve b shows the histogram of traumatised slices (n = 17). For a better view of the important section the y-axis was split at 12,000 and two different scales were used in both parts. The vertical dashed line is the applied threshold at a gray scale value of 75. The integral of all pixel values greater than the threshold was calculated for each group and defined as the trauma intensity. Two example images for (a) non-traumatised and (b) traumatised slices at t = 72 hours are shown in the upper right corner.