Figure 1

After 30 minutes of incubation with propidium iodide, baseline fluorescence imaging was performed on all slices to qualify the level of cell injury prior to trauma. Histograms were computed from images by counting the sum of all pixels sharing the same eight-bit gray scale value (from 0 to 255). (a) The mean with the standard error of the mean (SEM) of a total of 206 slices included in this setting is shown. (b) An enlarged portion of the graph around the applied threshold of 75 (dashed line) is shown. The continuous line is the mean value, and the dotted lines are the upper and lower bounds of the SEM. The data demonstrates the continuous low level of injury throughout the slices prior to traumatisation.