Anti-C5a ameliorates impairment of the blood-brain barrier after caecal ligation and puncture (CLP). (a-e) Brains were surgically removed, snap-frozen and tissue sections (10 μm) were obtained. Cerebral extravasation of rat albumin was assessed by immunohistochemistry 24 hours after CLP or sham procedure, three samples per experimental condition. Stains displayed are representative of three independent experiments. (f, g) Comparison of Evans Blue extravasation into the cerebellum and pituitary in IgG-treated or anti-C5a treated rats 24 hours post CLP. Displayed depictions are representative of four animals. (h, i) Quantification of Evans Blue extravasation into the brain or pituitary by determination of mg Evans Blue/mg tissue ratio in different groups at indicated time-points, four for each experimental condition. # p < 0.05 between sham and 24 hours CLP animals; * p < 0.05 between IgG-treated and anti-C5a-treated rats.