Figure 3
Hydrogen sulfide attenuation of oxidative DNA damage in the kidney after organ ischemia–reperfusion. Oxidative DNA damage (tail moment in the alkaline version of the comet assay [89]) in kidney tissue biopsies prior to (left panel) and after 2 hours of organ ischemia and 8 hours of reperfusion (right panel) in control swine (n = 7; open box plots) and in animals treated with the hydrogen sulfide donor sodium disulfide (Na2S) (n = 8; grey box plots). Renal ischemia was induced by inflating the balloon of an intra-aortic catheter positioned at the renal artery orifices. Na2S infusion was infused before kidney ischemia (2 mg/kg/hour over 2 hours) as well as during the first 4 hours of reperfusion (1 mg/kg/hour) [72]. Data presented as median (quartiles, range). #P < 0.05 versus before ischemia, §P < 0.05 versus control.