Phosphoinositide-3 kinase gamma kinase activity significantly contributes to the pathophysiology of sepsis and multiorgan failure

Table 1 Portion of results displaying the systemic, lung, liver and coagulation responses of PI3KγWT, KO and KD mice to CLP-induced sepsis

	WT sham	KO sham	KD sham	WT CLP	KO CLP	KD CLP
Systemic response
MIP-2 concentration (pg/ml in plasma × 10⁴)	0 ± 0	0 ± 0	0 ± 0	13 ± 5**	2 ± 1*	2 ± 1*
IL-6 concentration (pg/ml in plasma × 10³)	0 ± 0	0 ± 0	0 ± 0	18 ± 5*	19 ± 10*	22 ± 8*
Lung injury
Pathology score	1.67 ± 0.42	1.8 ± 0.49	1.71 ± 0.42	4.17 ± 0.31*	2.00 ± 0.63	2.00 ± 0.45
Permeability (protein in BAL (μg/ml))	194 ± 7	199 ± 3	195 ± 8	234 ± 13*	191 ± 13	192 ± 10
Neutrophil infiltration (cell × 10³/ml BAL)	0.3 ± 0.2	0.8 ± 0.4	0.2 ± 0.2	11.7 ± 5.9*	0.2 ± 0.1	0.8 ± 0.3
MIP-2 concentration (pg/ml in BAL × 10²)	0 ± 0	0 ± 0	0 ± 0	21.6 ± 1.0*	1.1 ± 0.4	1.2 ± 0.5
IL-6 concentration (pg/ml in BAL × 10²)	0 ± 0	0 ± 0	0 ± 0	14 ± 5*	1 ± 0	2 ± 0
Apoptosis (% apoptotic cells)	0.3 ± 0.1	1.2 ± 0.2*	1.0 ± 0.2*	7.7 ± 0.8**	1.3 ± 0.3*	1.2 ± 0.3*
Akt phosphorylation (fold increase over WT sham)	1.0 ± 0.1	0.7 ± 0.0*	0.7 ± 0.1*	1.4 ± 0.1**	0.8 ± 0.1*	0.8 ± 0.1*
Liver injury
Pathology score	0.33 ± 0.21	0.20 ± 0.20	0.20 ± 0.20	2.83 ± 0.17**	1.20 ± 0.37*	1.83 ± 0.17*
Apoptosis (% apoptotic cells)	1.0 ± 0.2	4.6 ± 1.2*	4.3 ± 1.0*	23.9 ± 2.7**	5.6 ± 0.7*	4.9 ± 1.0*
Coagulation
tPA (pg/ng protein)	404 ± 17	369 ± 53	423 ± 23	1 ± 1**	104 ± 36*	61 ± 31*
PAI-1 (pg/μg protein)	2.9 ± 0.2	2.7 ± 0.2	3.1 ± 0.4	84.8 ± 13.4**	50.5 ± 9.2*	52.0 ± 9.0*
Fibrinogen (ng/mg protein)	805 ± 35	807 ± 40	779 ± 33	1424 ± 89**	1043 ± 57*	1079 ± 58*

BAL, bronchoalveolar lavage; tPA, tissue plasminogen activator; PAI-1, plasminogen activator inhibitor 1.

ISSN: 1364-8535