Skip to main content

Table 1 Summary of the characteristics of the tested quantitation methods

From: Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid ‘real-time’ polymerase chain reaction

  Lower detection limit*      
    Log-linear range    Cost per test Set up
Method (CFU/g) (CFU/reaction) (CFU/g) Time to result (h) Ease of use (US$) cost ($)
Culture 102 1 102-109 24 Easy 6 3500
PCR 104-105 10-102 106-108 4 Moderate 12 20 000
ELISA-PCR 103-104 1-10 105-107 8 Elaborate 25 20 000
RTD-PCR (LC) 103-104 1-10 103-109 1 Moderate 9 73 000
  1. *The lower detection limit is variable due to variations in inter-run amplification efficiency. Not including labour costs, only reagents and disposables. Cost per clinical sample, based on the analysis of batches of 10 clinical samples and eight standards. LC, LightCycler.