Figure 8
PD98059, and Akt-deficient mice reduced high-tidal-volume-induced lung fibrosis. Representative photomicrographs (×100) with Masson's trichrome staining of paraffin lung sections obtained after 10 days of bleomycin treatment in wild-type (WT) mice or serine/threonine kinase-protein kinase B (Akt)+/- mice ventilated at a tidal volume (VT) of 6 ml/kg or 30 ml/kg for 5 hours with or without pretreatment with 2 mg/kg PD98059 subcutaneously for 30 minutes (n = 6 per group). (a) 6 ml/kg VT WT mice. (b) 30 ml/kg VT WT mice. (c) 30 ml/kg VT WT mice pretreated with PD98059. (d) 30 ml/kg VT Akt+/- mice. Peribronchiolar and parenchymal blue staining indicates positive staining for lung fibrosis. (e) Hydroxyproline (OH) contents were obtained from lung tissues of mice (n = 6 per group). (f) Fibrotic scoring was quantified as the average number of 10 nonoverlapping fields in Masson's trichrome staining of paraffin lung sections (n = 6 per group). *P < 0.05 versus control, nonventilated mice with bleomycin pretreatment; †P < 0.05 versus all other groups; ‡P < 0.05 versus control, nonventilated mice without bleomycin pretreatment.