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Figure 4 | Critical Care

Figure 4

From: Serine/threonine kinase-protein kinase B and extracellular signal-regulated kinase regulate ventilator-induced pulmonary fibrosis after bleomycin-induced acute lung injury: a prospective, controlled animal experiment

Figure 4

PD98059, SB203580, and Akt-deficient mice reduced high-tidal-volume-induced S100A4-positive fibroblast accumulation. Representative photomicrographs (×400) with S100A4 staining of paraffin lung sections via immunohistochemistry from 5 days of bleomycin treatment in wild-type (WT) mice or serine/threonine kinase-protein kinase B (Akt)+/- mice ventilated at a tidal volume (VT) of 6 ml/kg or 30 ml/kg for 5 hours with or without pretreatment with 2 mg/kg PD98059 or 16 mg/kg SB203580 subcutaneously for 30 minutes (n = 6 per group). (a) Control WT mice. (b) 6 ml/kg VT WT mice, (c) 30 ml/kg VT WT mice. (d) 30 ml/kg VT WT mice pretreated with PD98059. (e) 30 ml/kg VT WT mice pretreated with SB203580. (f) 30 ml/kg VT Akt+/- mice. A dark-brown diaminobenzidine signal indicates positive staining for S100A4 in the lung epithelium or interstitium, while shades of bluish tan signify nonreactive cells.

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Critical Care

ISSN: 1364-8535