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Figure 3 | Critical Care

Figure 3

From: Serine/threonine kinase-protein kinase B and extracellular signal-regulated kinase regulate ventilator-induced pulmonary fibrosis after bleomycin-induced acute lung injury: a prospective, controlled animal experiment

Figure 3

PD98059, SB203580, and Akt-deficient mice reduced high-tidal-volume-induced type III procollagen mRNA expression. Five days after bleomycin administration, wild-type mice or serine/threonine kinase-protein kinase B (Akt)+/- mice ventilated at a tidal volume (VT) of 6 ml/kg or 30 ml/kg for 1 hour were pretreated with 2 mg/kg PD98059 or 16 mg/kg SB203580 subcutaneously for 30 minutes. RT-PCR assay was performed for (a) type I and (b) type III procollagen mRNA (top panel), GAPDH mRNA (middle panel), and arbitrary units (bottom panel) (n = 6 per group). Arbitrary units expressed as the ratios of type I and type III procollagen mRNA to GAPDH. *P < 0.05 versus control, nonventilated mice with bleomycin pretreatment; †P < 0.05 versus all other groups; ‡P < 0.05 versus control, nonventilated mice without bleomycin pretreatment.

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