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Figure 2 | Critical Care

Figure 2

From: Serine/threonine kinase-protein kinase B and extracellular signal-regulated kinase regulate ventilator-induced pulmonary fibrosis after bleomycin-induced acute lung injury: a prospective, controlled animal experiment

Figure 2

PD98059, SB203580, and Akt-deficient mice reduced high-tidal-volume-induced lung fibrosis. Representative photomicrographs (×100) with Masson's trichrome staining of paraffin lung sections 5 days after bleomycin-treatment in wild-type (WT) mice or serine/threonine kinase-protein kinase B (Akt)+/- mice ventilated at a tidal volume (VT) of 6 ml/kg or 30 ml/kg for 5 hours with or without pretreatment with 2 mg/kg PD98059 or 16 mg/kg SB203580 subcutaneously for 30 minutes (n = 6 per group). (a) Control WT mice. (b) 6 ml/kg VT WT mice. (c) 30 ml/kg VT WT mice. (d) 30 ml/kg VT WT mice pretreated with PD98059. (e) 30 ml/kg VT WT mice pretreated with SB203580. (f) 30 ml/kg VT Akt+/- mice. Peribronchiolar and parenchymal blue staining indicates positive staining for lung fibrosis. (g) 30 ml/kg VT WT mice without pretreatment with bleomycin. (h) Fibrotic scoring was quantified as the average number of 10 nonoverlapping fields in Masson's trichrome staining of paraffin lung sections (n = 6 per group). *P < 0.05 versus control, nonventilated mice with bleomycin pretreatment; †P < 0.05 versus all other groups; ‡P < 0.05 versus control, nonventilated mice without bleomycin pretreatment.

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