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Figure 4 | Critical Care

Figure 4

From: High-molecular-weight hyaluronan – a possible new treatment for sepsis-induced lung injury: a preclinical study in mechanically ventilated rats

Figure 4

Hyaluronan (1,600 kDa and 35 kDa) inhibited cytokine mRNA expression. Animals were pretreated with either 1,600 kDa or 35 kDa hyaluronan 18 hours prior to ventilation. Lipopolysaccharide (LPS) (1 mg/kg) was given by arterial injection 1 hour prior to the start of ventilation. After 4 hours of ventilation, animals were sacrificed and the right upper lobe was flash frozen for extraction of RNA. Macrophage inflammatory protein-2 (MIP-2) and TNFα mRNA expression was decreased with high-molecular-weight hyaluronan or low-molecular-weight hyaluronan. (a) RT-PCR. (b) Quantitation of mRNA expression for MIP-2. (c) Quantitation of mRNA expression for TNFα. C, Control; L, LPS. *P < 0.05 versus control, #P < 0.05 versus LPS.

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Critical Care

ISSN: 1364-8535