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Table 1 PCR and pyrosequencing primers

From: Association of HMGB1 polymorphisms with outcome in patients with systemic inflammatory response syndrome

Genetic variant

PCR

Pyrosequencing primer

 

Primer set

Forward primer

Reverse primer

 

Sequencing

    

   -1615A>G

A

5'-ATG TGC ATG TGT GAT ATA TTG TCC-3'

5'-GTT ATA TCA GTG CTT TAT GAA ACT AC-3'

 

   -1377delA

    

   1747delT

B

5'-CAA AGT TTT ATG CAA GGA GGG TG-3'

5'-GTC CAT TCA GGG CGA TCT C-3'

 

   1779T>G

    

   1808C>G

    

   1822G>A

    

   1888insT

    

   4519_4521delGAT

C

5'-AAA GTT CTG CCA TGT TCT ATT TC-3'

5'-CAG GAC AGG GCT ATC TAA AG-3'

 

   2351insT

    

Pyrosequencing

    

   -196C>A

 

5'-CTC TTT GCC CGG CAT ACA CA-3'

5'-TCC TGA CCA GAG CCC GTT T-3'

5'-TTG ATG ACG TGT CCC-3'

   3814C>G

 

5'-GTC TGA TTT TAC GGA GGT TGA-3'

5'-CCT TTG CCC ATG TTT AGT TAT T-3'

5'-TAC TTT GGT TTT CAT TCC-3'

   982C>T

 

5'-TGT TCA TCT AGG GTT CTA GCT-3'

5'-CCT TTG ATT TTT GGG CGA TAC-3'

5'-TTA GTT CGG CCT TCT T-3'

   1177G>C

 

5'-AAC TGG GAG AGA TGT GGA ATA-3'

5'-CAA TCA TAC ATC TGG CGT ACT-3'

5'-GGT TTG CTT GGT AAA ATG-3'

  1. Forward primers from primer sets A, B and C contain a 5'-T7 sequence (5'-TAA TAC GAC TCA CTA TAG GG-3'). The 5' ends of all of the PCR reverse primers used for pyrosequencing are biotinylated.