Figure 5From: Exogenous nitric oxide requires an endothelial glycocalyx to prevent postischemic coronary vascular leak in guinea pig heartsImmunostaining of microvessels of guinea pig hearts with monoclonal antibody against heparan sulfate after minimal perfusion (a, b), after 15 minutes of ischemia followed by 20 minutes of reperfusion in the absence (c, d) and presence (g, h) of nitric oxide, and after the glycocalyx had been enzymatically degraded by means of heparinase (e, f). Specimens are paraffin-embedded sections of guinea pig heart. Original magnifications: × 20 (lefthand panels) and × 100 (righthand panels). HS, heparan sulfate; VL, vascular lumen.Back to article page