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Figure 1 | Critical Care

Figure 1

From: Exogenous nitric oxide requires an endothelial glycocalyx to prevent postischemic coronary vascular leak in guinea pig hearts

Figure 1

Experimental protocols. After heart preparation and an equilibration period of 15 minutes, hearts were subjected to 15 minutes of global normothermic ischemia followed by 20 minutes of reperfusion. Hearts were reperfused in the absence (Control group) and presence (NO group) of 4 μM nitric oxide. In two further series, the glycocalyx was enzymatically degraded by heparinase, applied into the coronary system in the course of ischemia, followed again by reperfusion in the absence (HEP group) and presence (HEP+NO group) of 4 μM NO. Transudate and coronary effluent were quantified at baseline (B) (just before ischemia) and at 1, 2, 3, 4, 5, 8, 15, and 20 minutes of reperfusion. HEP, heparinase.

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Critical Care

ISSN: 1364-8535