Changes in high mobility group box 1 (HMGB1) protein expression in lung tissue after lipopolysaccharide (LPS) administration in rats. (a) The expression of HMGB1 protein in the lung 12 h following administration of LPS in untreated LPS and danaparoid sodium (DA)-treated LPS groups was detected by Western blot. Representative blots from three separate experiments are shown. (b) Signal intensities for HMGB1 expression in lung tissue were quantified using an image analyser. Black bars represent the negative control group, white bars represent the LPS group, mesh bars represent the DA-treated LPS group. The expression intensity of HMGB1 protein relative to that of the negative control group was calculated for each group.