Figure 7
From: Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo
Effects OxPAPC on barrier properties in macro- and microvascular pulmonary endothelial cells (ECs). Human pulmonary artery (HPAEC) or microvascular (HLMVEC) ECs were plated on gold microelectrodes to measure transendothelial electrical resistance (TER) and were cultured to confluence. Growth medium was replaced with serum-free Opti-MEM (Invitrogen Corporation, Carlsbad, CA, USA). After equilibration and stabilization, measurements of TER were performed. At the time indicated by the arrow, ECs were treated with various concentrations of OxPAPC (a) or cells were pretreated with OxPAPC (20 μg/mL) followed by thrombin (0.5 U/mL) stimulation (marked by second arrow) (b). Results are representative of three to six independent experiments. OxPAPC, oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine; Thr, thrombin.