Figure 3
From: Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo
Histological assessment of the effect of OxPAPC on ventilator-induced lung injury. Whole lungs (4 to 6 animals from each experimental group) were agarose-inflated in situ, fixed in 10% formalin, and used for histologic evaluation by hematoxylin and eosin staining as described in Materials and methods. Histological analysis of lung tissue (×40 magnification) (a) and quantitative analysis of lung tissue neutrophil count (b) obtained from rats exposed to high tidal volume (HTV) mechanical ventilation demonstrate a neutrophilic inflammation and areas of alveolar hemorrhage, which were attenuated by co-treatment with intravenous OxPAPC. For tissue polymorphonuclear leukocyte (PMN) counts, 10 fields per slide were counted for n = 4 animals per experimental group. *p < 0.01 versus low tidal volume (LTV), **p < 0.05 versus HTV (n = 4 to 6 per group). HPF, high power microscopic field; OxPAPC, oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine.