Skip to main content
Figure 2 | Critical Care

Figure 2

From: Biphasic onset of splenic apoptosis following hemorrhagic shock: critical implications for Bax, Bcl-2, and Mcl-1 proteins

Figure 2

Hemorrhagic shock (HS)-induced apoptosis of murine spleen. (a) DNA fragmentation as shown by TUNEL staining. Splenocytes were isolated from HS and sham animals at t = 0 hours, t = 24 hours, and t = 72 hours after hemorrhage as well as from control animals. Results are representative of at least three animals per group and controls. (b) Quantificational analysis of DNA fragmentation. Results are representative of at least three animals per group and controls. (c) Activity of death-receptor-mediated effector caspase-3/7 and caspase-8 as well as mitochondria-related caspase-9 activity within the entire observation period. *P < 0.05 as determined by analysis of variance (with post hoc Bonferroni/Dunn) test and Mann-Whitney test. Co, control; RFU, relative fluorescent units; RLU, relative light units; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling.

Back to article page

Critical Care

ISSN: 1364-8535