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Figure 8 | Critical Care

Figure 8

From: Platelet-derived exosomes induce endothelial cell apoptosis through peroxynitrite generation: experimental evidence for a novel mechanism of septic vascular dysfunction

Figure 8

Platelet-derived exosomes possess NADPH oxidase and nitric oxide synthases. Representative Western blot images of exosomes from different origins (septic platelets (sepsis), platelets exposed to diethylamine-NONOate (NONO), lipopolysaccharide (LPS), TNF-α, thrombin (Thr) and saline (Ctl)) were subjected to SDS-PAGE and exposed to antibodies directed to the different nitric oxide synthase (NOS) isoforms: neuronal (nNOS), inducible (iNOS) and endothelial (eNOS), to the NADPH oxidase regulatory protein protein disulfide isomerase (PDI), to the NADPH oxidase membrane-bound subunit isoforms Nox 1 and Nox2, and to the NADPH oxidase membrane component p22phox. Leukocytes were used as positive controls for iNOS, and NADPH oxidase components, endothelial cells activated (+) or not (-) with LPS were used as controls for eNOS/iNOS expression. Results shown are representative of at least three different experiments.

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Critical Care

ISSN: 1364-8535