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Figure 3 | Critical Care

Figure 3

From: Platelet-derived exosomes induce endothelial cell apoptosis through peroxynitrite generation: experimental evidence for a novel mechanism of septic vascular dysfunction

Figure 3

Lucigenin luminescence: exosomes from platelets exposed to NO or LPS are similar to septic exosomes. The graph represents NADPH-dependent lucigenin (5 μM) chemiluminescence above background. Exosomes (10 μg protein content) obtained from platelets exposed to the nitric oxide donor diethylamine NONOate (NONOate; 0.5 μM) or lipopolysaccharide (LPS; 100 ng/ml) generate reactive oxygen species in a similar fashion to exosomes obtained from septic patients, whereas particles obtained from platelets exposed to saline (control) or thrombin (5 IU/ml) have very low activity. For comparison, luminescence obtained with platelets from healthy (control) and septic subjects are displayed. Results normalized for sample protein concentration are means ± SD of three or more experiments. *P < 0.05 versus control.

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Critical Care

ISSN: 1364-8535